Four electrode materials: Pt, Au, Pd and glassy carbon (GC), were studied to investigate their suitability as substrates in the development of two different classes of glutamate biosensor. Glutamate oxidase cross-linked onto poly(o-phenylenediamine) was chosen as the type 1 biosensor (PPD/GluOx), incorporating PPD as the permselective element to detect H2O2 directly on the electrode surface at relatively high applied potentials. GluOx and horseradish peroxidase/redox polymer modified electrodes (Os2+PVP/HRP/GluOx) that relied on enzyme-catalysed H2O2 detection at lower applied potentials were used as type 2 biosensors. The voltammetric and amperometric responses to the enzyme signal transduction molecule, H2O2, and the archetypal interference species in biological applications, ascorbic acid, were determined on the bare and PPD/GluOx-modified surfaces. The amperometric responses of these electrodes were stable over several days of continuous recording in phosphate buffered saline (pH 7.4). The sensitivity of the type 1 biosensors to H2O2 and glutamate showed parallel trends with low limits of detection and good linearity at low concentrations: Pt>Au∼Pd⪢GC. Type 2 biosensors out-performed the type 1 design for all electrode substrates, except Pt. However, the presence of the permselective PPD membrane in the type 1 biosensors, not feasible in the type 2 design, suggests that Pt/PPD/GluOx might have the best all-round characteristics for glutamate detection in biological media containing interference species such as ascorbic acid. Other points affecting a final choice of substrate should include factors such as mass production issues.